Edward R. Seidel, Ph.D. Professor      Physiology Department Brody    6N-59 Phone    252.744.2775 FAX    252.744.3460 seidele@ecu.edu

 

1980

Ph.D.,University of Alabama in Birmingham

 

Address

 

Dr. Edward R. Seidel

EastCarolinaUniversity

BrodySchool of Medicine

Physiology Department

600 Moye Blvd.

6N-59 Brody Bldg.

Greenville, NC  27834

 

Research Interests

 

Physiology Research Program summary

The polyamines are a group of ubiquitously distributed organic polycations that are intimately involved in both normal and abnormal cellular proliferation. My laboratory is studying the mechanism by which polyamines exert their effects on proliferation. We have shown that polyamines regulate expression of the gene encoding the translational repressor 4E binding protein 1. By regulating 4E binding protein 1 polyamines regulate cap-dependent mRNA translation. The cap-dependent translation complex is responsible for translation of 80% of all mRNA transcripts thus polyamines modulate translation of a host of factors involved in cell proliferation including various oncogenes, transcription factors, and growth factors. A second area of interest in the laboratory involves isolating one of the cell membrane polyamine transport proteins. Our data indicate that polyamines may be transported through one of the y+ class of amino acid transporters. The CHO-MG cell is a polyamine transport deficient cell line. Experiments are underway to produce a stable transfectant expressing the amino acid transport protein in the CHO-MG cell. The results of these experiments will determine whether polyamines are transported via an amino acid transporter.

 

Recent Publications

 

• Gill JE, Christian JF and Seidel ER. Antizyme mRNA distribution and regulation in rat small intestinal enterocytes. Digestive Diseases and Sciences 47(7):1458-1464, 2002.
  
• Seidel ER, Regan V and Liu L. Nitration of tyrosine residues by peroxynitrite inhibits the activity of ornithine decarboxylase. Life Sciences 68:1477-1483, 2001.
  
• Brachet P, Long J and Seidel ER. Selective sites for polyamine binding to rabbit intestinal brush border membranes. Biochemical Pharmacology 55(12):517-526, 1998.
  
• Seidel ER and Regan VL. Inhibition by rapamycin of ornithine decarboxylase and epithelial cell proliferation in intestinal epithelial cells in culture. Brit J Pharmacol 120:571-574, 1997.
  
• Seidel ER and Scemama J -L. Gastrointestinal polyamines and regulation of mucosal growth and function. Journal of Nutritional Biochemistry 8:104-111, 1997 (Invited review).